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pbs egfp okuda  (Addgene inc)


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    Addgene inc pbs egfp okuda
    Pbs Egfp Okuda, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs egfp okuda/product/Addgene inc
    Average 94 stars, based on 1 article reviews
    pbs egfp okuda - by Bioz Stars, 2026-05
    94/100 stars

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    Functional validation of <t>EVI2B</t> in steatosis models and its pro-steatotic role. (A) Representative histopathological images (H&E staining) of liver sections from mice fed an HFD for 17 weeks, showing severe hepatic steatosis compared to chow diet controls. (B) RT-qPCR analysis of candidate gene expression (PRF1, EVI2B, CST7, GNG2, KLHL24) in livers of HFD-fed mice versus controls (* p < 0.05, ** p < 0.01, *** p < 0.001). (C-D) Consistent upregulation of PRF1, EVI2B, CST7, GNG2, KLHL24, and related genes in livers of ob/ob mice (C: mRNA levels). (E-F) Construction of Evi2b-overexpressing lentivirus: validation of Evi2b overexpression in hepatocytes (E) mRNA and (F)protein. (G) Oil Red O staining of AML12 and primary hepatocytes overexpressing Evi2b, demonstrating exacerbated lipid droplet accumulation (scale bar = 20 μm).
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    Functional validation of <t>EVI2B</t> in steatosis models and its pro-steatotic role. (A) Representative histopathological images (H&E staining) of liver sections from mice fed an HFD for 17 weeks, showing severe hepatic steatosis compared to chow diet controls. (B) RT-qPCR analysis of candidate gene expression (PRF1, EVI2B, CST7, GNG2, KLHL24) in livers of HFD-fed mice versus controls (* p < 0.05, ** p < 0.01, *** p < 0.001). (C-D) Consistent upregulation of PRF1, EVI2B, CST7, GNG2, KLHL24, and related genes in livers of ob/ob mice (C: mRNA levels). (E-F) Construction of Evi2b-overexpressing lentivirus: validation of Evi2b overexpression in hepatocytes (E) mRNA and (F)protein. (G) Oil Red O staining of AML12 and primary hepatocytes overexpressing Evi2b, demonstrating exacerbated lipid droplet accumulation (scale bar = 20 μm).
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    Functional validation of EVI2B in steatosis models and its pro-steatotic role. (A) Representative histopathological images (H&E staining) of liver sections from mice fed an HFD for 17 weeks, showing severe hepatic steatosis compared to chow diet controls. (B) RT-qPCR analysis of candidate gene expression (PRF1, EVI2B, CST7, GNG2, KLHL24) in livers of HFD-fed mice versus controls (* p < 0.05, ** p < 0.01, *** p < 0.001). (C-D) Consistent upregulation of PRF1, EVI2B, CST7, GNG2, KLHL24, and related genes in livers of ob/ob mice (C: mRNA levels). (E-F) Construction of Evi2b-overexpressing lentivirus: validation of Evi2b overexpression in hepatocytes (E) mRNA and (F)protein. (G) Oil Red O staining of AML12 and primary hepatocytes overexpressing Evi2b, demonstrating exacerbated lipid droplet accumulation (scale bar = 20 μm).

    Journal: Scientific Reports

    Article Title: Immune metabolic changes identify causal candidate genes and enable diagnostic frameworks in MAFLD

    doi: 10.1038/s41598-025-17406-2

    Figure Lengend Snippet: Functional validation of EVI2B in steatosis models and its pro-steatotic role. (A) Representative histopathological images (H&E staining) of liver sections from mice fed an HFD for 17 weeks, showing severe hepatic steatosis compared to chow diet controls. (B) RT-qPCR analysis of candidate gene expression (PRF1, EVI2B, CST7, GNG2, KLHL24) in livers of HFD-fed mice versus controls (* p < 0.05, ** p < 0.01, *** p < 0.001). (C-D) Consistent upregulation of PRF1, EVI2B, CST7, GNG2, KLHL24, and related genes in livers of ob/ob mice (C: mRNA levels). (E-F) Construction of Evi2b-overexpressing lentivirus: validation of Evi2b overexpression in hepatocytes (E) mRNA and (F)protein. (G) Oil Red O staining of AML12 and primary hepatocytes overexpressing Evi2b, demonstrating exacerbated lipid droplet accumulation (scale bar = 20 μm).

    Article Snippet: The coding sequence of murine Evi2b (NCBI Gene ID: 216984) was cloned into the lentiviral vector lentiCas9-EGFP (Addgene, #85400) to replace EGFP, generating Lenti-Evi2b.

    Techniques: Functional Assay, Biomarker Discovery, Staining, Quantitative RT-PCR, Gene Expression, Over Expression